| Gene Set | Qualifiers | Information Source | Evidence | Pentacon Note |
|---|---|---|---|---|
| AAP | Likely | Expert advice: Robert Murphy, Ph.D.; Literature |
10578051|E |
ACOT7 had very low activity with arachidonyl-CoA, but had greater activity with other long chain acyl-CoAs, including oleoyl-CoA (10578051) |
| Function | Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May play an important physiological function in brain. May play a regulatory role by modulating the cellular levels of fatty acyl-CoA ligands for certain transcription factors as well as the substrates for fatty acid metabolizing enzymes, contributing to lipid homeostasis. Has broad specificity, active towards fatty acyl-CoAs with chain-lengths of C8-C18. Has a maximal activity toward palmitoyl-CoA. UniProt |
| Isoforms |
7 isoform(s):
Isoform 1;
Isoform 2;
Isoform 3;
Isoform 4;
Isoform 5;
Isoform 6;
Isoform 7;
UniProt |
| Natural variants | |
| Mutagenesis sites | |
| Tissues/Cell Types |
brain [BTO:0000142];
UniProt |
| Localization | |
| Diseases | |
| Orthologs | Acot7 (Mus musculus)i, Acot7 (Rattus norvegicus)i, acot7 (Danio rerio)i, |
| Pentacon Resources | AAP Figure, NSAIDnet, GIANT |
| Protein and Genetic Interaction: | BioGRID |
| CRISPR Screens | BioGRID ORCS CRISPR Screen Phenotypes |
| Data Type | Value | ChEBI Term A | ChEBI Term B | 1° Note | 1° Source | 2° Source |
|---|---|---|---|---|---|---|
| KM | 0.0092 mM | icosanoyl-CoA [CHEBI:15527] | <52> Yamada, J.; Kurata, A.; Hirata, M.; Taniguchi, T.; Takama, H.; Furihata, T.; Shiratori, K.; Iida, N.; Takagi-Sakuma, M.; Watanabe, T.; Kurosaki, K.; Endo, T.; Suga, T.: Purification, molecular cloning, and genomic organization of human brain long-chain acyl-CoA hydrolase. J. Biochem. (1999) 126, 1013-1019. {Pubmed:10578051} | PubMed:10578051 | Brenda | |
| KM | 0.0076 mM | lauroyl-CoA [CHEBI:15521] | <52> Yamada, J.; Kurata, A.; Hirata, M.; Taniguchi, T.; Takama, H.; Furihata, T.; Shiratori, K.; Iida, N.; Takagi-Sakuma, M.; Watanabe, T.; Kurosaki, K.; Endo, T.; Suga, T.: Purification, molecular cloning, and genomic organization of human brain long-chain acyl-CoA hydrolase. J. Biochem. (1999) 126, 1013-1019. {Pubmed:10578051} | PubMed:10578051 | Brenda | |
| KM | 0.014 mM | octanoyl-CoA [CHEBI:15533] | <52> Yamada, J.; Kurata, A.; Hirata, M.; Taniguchi, T.; Takama, H.; Furihata, T.; Shiratori, K.; Iida, N.; Takagi-Sakuma, M.; Watanabe, T.; Kurosaki, K.; Endo, T.; Suga, T.: Purification, molecular cloning, and genomic organization of human brain long-chain acyl-CoA hydrolase. J. Biochem. (1999) 126, 1013-1019. {Pubmed:10578051} | PubMed:10578051 | Brenda | |
| KM | 0.0088 mM | oleoyl-CoA [CHEBI:15534] | <52> Yamada, J.; Kurata, A.; Hirata, M.; Taniguchi, T.; Takama, H.; Furihata, T.; Shiratori, K.; Iida, N.; Takagi-Sakuma, M.; Watanabe, T.; Kurosaki, K.; Endo, T.; Suga, T.: Purification, molecular cloning, and genomic organization of human brain long-chain acyl-CoA hydrolase. J. Biochem. (1999) 126, 1013-1019. {Pubmed:10578051} | PubMed:10578051 | Brenda | |
| KM | 0.0064 mM | palmitoyl-CoA [CHEBI:15525] | <52> Yamada, J.; Kurata, A.; Hirata, M.; Taniguchi, T.; Takama, H.; Furihata, T.; Shiratori, K.; Iida, N.; Takagi-Sakuma, M.; Watanabe, T.; Kurosaki, K.; Endo, T.; Suga, T.: Purification, molecular cloning, and genomic organization of human brain long-chain acyl-CoA hydrolase. J. Biochem. (1999) 126, 1013-1019. {Pubmed:10578051} | PubMed:10578051 | Brenda | |
| Vmax | 270000.0 nmol/min/mg | icosanoyl-CoA [CHEBI:15527] | Added by PENTACON; PENTACON Notes: pH 7.4, 30°C | PubMed:10578051 | Pentacon | |
| Vmax | 295000.0 nmol/min/mg | lauroyl-CoA [CHEBI:15521] | Added by PENTACON; PENTACON Notes: pH 7.4, 30°C | PubMed:10578051 | Pentacon | |
| Vmax | 269000.0 nmol/min/mg | octanoyl-CoA [CHEBI:15533] | Added by PENTACON; PENTACON Notes: pH 7.4, 30°C | PubMed:10578051 | Pentacon | |
| Vmax | 50000.0 nmol/min/mg | oleoyl-CoA [CHEBI:15534] | Added by PENTACON; PENTACON Notes: pH 7.4, 30°C | PubMed:10578051 | Pentacon | |
| Vmax | 275000.0 nmol/min/mg | palmitoyl-CoA [CHEBI:15525] | Added by PENTACON; PENTACON Notes: pH 7.4, 30°C | PubMed:10578051 | Pentacon |
